LOCUS       pTriEx-1.1   5301 bp    DNA   circular            16-OCT-2002
DEFINITION  pTriEx-1 replacement.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|189525593|
COMMENT     VNTDBDATE|280683239|
COMMENT     VNTNAME|pTriEx-1.1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Keith W. Yaeger|
COMMENT     VNTOAUTHORTEL|(608) 238-6110  x133|
COMMENT     VNTOAUTHORFAX|(608) 238-1388|
COMMENT     VNTOAUTHOREML|keith.yaeger@novagen.com|
COMMENT     VNTOAUTHORAD1|Novagen|
COMMENT     VNTOAUTHORAD2|601 Science Dr.|
COMMENT     VNTOAUTHORAD3|Madison,  WI  53711|
COMMENT     VNTOAUTHORAD4|U.S.A.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         27 28 29 30 31 92 32 34 35 33 53 58 93 56 94 37 57 38 36 39 40 41 42 43 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2363 0) 
COMMENT        #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2409 0) 
COMMENT        #6=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 2332 0) 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2389 0) 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 2346 0) 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 2344 0)) 
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COMMENT             (CStringList) 1 1 1 1 "3572..3833") 
COMMENT        #13=(CFSignal (CObList) "Exon 3 (Rabbit Globin)" 61 0 0 2082 2149 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "151..218") 
COMMENT        #14=(CFSignal (CObList) "Rabbit B-Globin Poly A" 25 0 0 2518 2800 0 
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COMMENT        #15=(CFSignal (CObList) "1629" 87 0 0 2864 3569 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "COLE1_ORI" 33 0 1 3780 3780 0 (CStringList) 
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COMMENT                          "pTriEx-1 replacement" 1) 
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COMMENT                          "Currently local object. Original author: Keith W. Yaeger" 
COMMENT                          1) 
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COMMENT                          "Created: 03/17/00 01:59PM" 1) 
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COMMENT                          "Last Modified: 10/16/02 03:33PM" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5301 bp" 1) 
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COMMENT                          "TEL: (608) 238-6110  x133  FAX: (608) 238-1388" 1) 
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FEATURES             Location/Qualifiers
     enhancer        1079..1443
                     /vntifkey="9"
                     /label=CMV\Enhancer
     promoter        1449..1726
                     /vntifkey="29"
                     /label=Chicken\B-Actin\Promoter
     enhancer        45..1011
                     /vntifkey="9"
                     /label=Lef2,603
     exon            2082..2149
                     /vntifkey="61"
                     /label=Exon\3\(Rabbit\Globin)
     polyA_signal    2518..2800
                     /vntifkey="25"
                     /label=Rabbit\B-Globin\Poly\A
     misc_signal     2864..3569
                     /vntifkey="87"
                     /label=1629
     rep_origin      complement(3780..3780)
                     /vntifkey="33"
                     /label=COLE1_ORI
     CDS             complement(4371..5239)
                     /vntifkey="4"
                     /label=AmpR
     misc_feature    2441..2476
                     /vntifkey="21"
                     /label=HSV-Tag
     misc_feature    2483..2506
                     /vntifkey="21"
                     /label=His-Tag
     promoter        2150..2166
                     /vntifkey="30"
                     /label=T7\Promoter
     protein_bind    2171..2191
                     /vntifkey="31"
                     /label=lac\Operator
     terminator      2804..2851
                     /vntifkey="43"
                     /label=T7_TERM
     promoter        2205..2318
                     /vntifkey="29"
                     /label=p10\promoter\+\5'\UTR
     misc_feature    2338..2353
                     /vntifkey="21"
                     /label=3-ORF\Site
     misc_feature    2423..2438
                     /vntifkey="21"
                     /label=3-ORF\Site
BASE COUNT     1339 a      1232 c      1226 g      1504 t 
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg 
     1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct 
     1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 
     1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 
     1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 
     1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 
     1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 
     1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 
     1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 
     1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 
     1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 
     1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 
     1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg 
     1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 
     1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta 
     1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 
     1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc 
     1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 
     2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 
     2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc 
     2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc 
     2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat 
     2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatggcgat 
     2341 atcccgggag ctcgtggatc cgaattctca gatctcggcg cgcctgcagg tcgacggtac 
     2401 cggttcgaag cttgcggccg cacagctgta tacacgtgca agccagccag aactcgcccc 
     2461 ggaagacccc gaggatctcg agcaccacca tcaccatcac catcactaag tgattaacct 
     2521 caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa 
     2581 taccactgag atcgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct 
     2641 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa 
     2701 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa 
     2761 tgagtatttg gtttagagtt tggcaacata tgcccatatg taactagcat aaccccttgg 
     2821 ggcctctaaa cgggtcttga ggggtttttt gctgaaagca tgcggaggaa attctccttg 
     2881 aagtttccct ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc 
     2941 cggcgtatta aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc 
     3001 gttgttgatt tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt 
     3061 tagggtggta tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa 
     3121 atattaaatc ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt 
     3181 tttccgaacc gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca 
     3241 aatcttgtag cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag 
     3301 gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt 
     3361 acaattgact cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt 
     3421 tagctttatt aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg 
     3481 aagacgattt tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga 
     3541 tcaaatttgt agttgagctt tttggaattg cgatcgcata acttcgtata gcatacatta 
     3601 tacgaagtta taagctcgga acgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     3661 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     3721 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     3781 tccataggct ccgccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 
     3841 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 
     3901 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 
     3961 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 
     4021 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 
     4081 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 
     4141 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 
     4201 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 
     4261 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 
     4321 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgtt 
     4381 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     4441 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     4501 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     4561 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     4621 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     4681 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     4741 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     4801 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     4861 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     4921 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     4981 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     5041 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     5101 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     5161 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     5221 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     5281 attgtctcat gtccgcgcgt t 
//
