LOCUS       Exported                5450 bp ds-DNA     circular SYN 03-JUL-2023
DEFINITION  pTriEx-1 replacement.
ACCESSION   .
VERSION     .
KEYWORDS    pQBMam
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, July 3, 2023 from SnapGene 3.2.1
            http://www.snapgene.com
COMMENT     http://www.informaxinc.com/
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1021..1232
                     /organism="Vesiculovirus indiana"
                     /lab_host="BHK-21 cells"
                     /strain="Mudd-Summers"
                     /mol_type="viral cRNA"
                     /country="USA"
                     /db_xref="taxon:1972577"
     source          1233..1280
                     /organism="Vesiculovirus indiana"
                     /lab_host="BHK-21 cells"
                     /strain="Mudd-Summers"
                     /mol_type="viral cRNA"
                     /country="USA"
                     /db_xref="taxon:1972577"
     source          1295..1408
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     enhancer        45..1011
                     /label=Lef2,603
                     /note="Lef2,603"
     CDS             complement(1021..1280)
                     /codon_start=1
                     /product=" Gene Therapy (2006) 13, 304¨C312 "
                     /note="VSV-GED"
                     /translation="MKCLLYLAFLFIGVNCGDTGLSKNPIEFVEGWFSSWKSSIASFFF
                     TIGLIIGLFLVLRVGIYLCIKLKHTKKRQIYTDIEMNRLGK"
     misc_feature    1023..1109
                     /note="CTD"
     misc_feature    1110..1169
                     /note="TM"
     misc_feature    1170..1232
                     /note="GS"
     misc_feature    1233..1280
                     /note="sp"
     misc_feature    complement(1295..1408)
                     /note="p10 promoter and 5' UTR"
     gene            complement(1367..1408)
                     /gene="Ac-p26"
                     /gene_synonym="AcOrf-136"
                     /note="Ac-p26"
     enhancer        1409..1768
                     /label=CMV Enhancer
                     /note="CMV Enhancer"
     promoter        1774..2051
                     /label=Chicken B-Actin Promoter
                     /note="Chicken B-Actin Promoter"
     exon            2407..2474
                     /label=Exon 3 (Rabbit Globin)
                     /note="Exon 3"
     misc_feature    2476..2485
                     /note="Kozak"
     misc_feature    2480..2661
                     /note="MCS"
     misc_feature    2590..2625
                     /label=HSV-Tag
                     /note="HSV-Tag"
     misc_feature    2632..2655
                     /label=His-Tag
                     /note="His-Tag"
     polyA_signal    2667..2949
                     /label=Rabbit B-Globin Poly A
                     /note="Rabbit B-Globin Poly A"
     misc_signal     3013..3718
                     /label=1629
                     /note="1629"
     rep_origin      complement(3929)
                     /direction=LEFT
                     /label=COLE1_ORI
                     /note="COLE1_ORI"
     CDS             complement(4528..5388)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
     1021 tactttccaa gtcggttcat ctctatgtct gtataaatct gtcttttctt ggtgtgcttt
     1081 aacttgatgc agaggtagat accaactcgg agaaccaaga atagtccaat gattaaccct
     1141 atggtgaaga agaagctggc aatagagctc ttccaactac tgaaccaacc ttctacaaac
     1201 tcgattggat tcttggatag cccagtatca ccgcaattca ccccgatgaa taaaaaagct
     1261 aagtacaaaa ggcacttcat ggtatatctc ctttgattgt aaataaaatg taatttacag
     1321 tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa ctataatata
     1381 ttgtgttggg ttgaattaaa ggtcccggta ttaatagtaa tcaattacgg ggtcattagt
     1441 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     1501 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     1561 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     1621 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     1681 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     1741 ctacgtatta gtcatcgcta ttaccatgca tggtcgaggt gagccccacg ttctgcttca
     1801 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
     1861 tttgtgcagc gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg
     1921 gcgaggggcg gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc
     1981 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     2041 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
     2101 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     2161 gcccttctcc ttcgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     2221 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     2281 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     2341 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     2401 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     2461 gatcggaccg aaattgccac catggcgata tcccgggagc tcgtggatcc gaattctcag
     2521 atctcggcgc gcctgcaggt cgacggtacc ggttcgaagc ttgcggccgc acagctgtat
     2581 acacgtgcaa gccagccaga actcgccccg gaagaccccg aggatctcga gcaccaccat
     2641 caccatcacc atcactaagt gattaacctc aggtgcaggc tgcctatcag aaggtggtgg
     2701 ctggtgtggc caatgccctg gctcacaaat accactgaga tcgatctttt tccctctgcc
     2761 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     2821 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     2881 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     2941 gcccatatgt aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     3001 ctgaaagcat gcggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt
     3061 tgcaccagac gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta
     3121 gtacatttat taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta
     3181 ttttaataat tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg
     3241 attttcagcg tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag
     3301 gtttcgatta gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg
     3361 attttcgctc aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat
     3421 attcgtttgt gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt
     3481 tgtatttctt tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaatag
     3541 agcttggaca tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc
     3601 ccaaccctcg tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct
     3661 attaattgtg tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattgc
     3721 gatcgcataa cttcgtatag catacattat acgaagttat aagctcggaa cgctgcgctc
     3781 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3841 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3901 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgccccctga cgagcatcac
     3961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     4141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4501 aaaaaaggat ctcaagaaga tcctttgtta ccaatgctta atcagtgagg cacctatctc
     4561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5401 tcaatattat tgaagcattt atcagggtta ttgtctcatg tccgcgcgtt
//
