LOCUS       Exported                4117 bp ds-DNA     circular SYN 21-MAY-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pQB4dual
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, May 21, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1295..1297
                     /organism="Bombyx mori"
                     /chromosome="14"
                     /strain="p50T"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:7091"
     source          1299..1360
                     /organism="Apis mellifera"
                     /strain="DH4"
                     /mol_type="genomic DNA"
                     /country="USA"
                     /isolation_source="bee hive"
                     /collection_date=2003
                     /sex="male"
                     /tissue_type="whole body"
                     /db_xref="taxon:7460"
     source          1361..1470
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     enhancer        45..1011
                     /note="Lef2,603"
     misc_feature    1025..1230
                     /note="p10 3'UTR"
     polyA_signal    1063..1068
                     /note="2nd PAS"
     polyA_signal    1161..1166
                     /note="1st PAS"
     CDS             complement(1241..1264)
                     /codon_start=1
                     /note="8xHis"
                     /translation="HHHHHHHH"
     sig_peptide     complement(1298..1360)
                     /gene="Melt"
                     /note="Melt signal peptide"
                     /inference="COORDINATES:same species:SignalP"
     promoter        complement(1361..1470)
                     /note="p10 promoter"
     misc_feature    1430
                     /note="TSS"
     gene            complement(1433..1470)
                     /gene="Ac-p26"
                     /gene_synonym="AcOrf-136"
                     /note="Ac-p26"
     promoter        1536..1627
                     /note="polh promoter"
     misc_feature    1578
                     /note="TSS"
     misc_feature    1628
                     /note="+1"
     CDS             1650..1712
                     /codon_start=1
                     /note="BmNPV gp64 signal peptide (LC068835.1)"
                     /translation="MVGAIVLYVLLAAAVPHSAFA"
     CDS             1749..1772
                     /codon_start=1
                     /note="8xHis"
                     /translation="HHHHHHHH"
     misc_signal     1781..2385
                     /note="1629"
     polyA_signal    2110..2115
                     /note="PAS"
     rep_origin      complement(2596)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(3187..4055)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggc
     1021 atgcaacaaa gaaaacaaca attttaatca aataatcatg ggtttatttg caacaaacac
     1081 aattgtggcg tttacagcat ttgttataca cacagaactc atatttttta tatttttcgt
     1141 aatcttccta atgatcacat tttattacat aatcaaagaa tcgtacgaat attataaaac
     1201 aattgatttg ttattttaaa aacgattcat gctcagctta gtgatggtga tggtgatggt
     1261 ggtgtccgga gaattcggta cccggccgga gctcgctagc atagatgtaa gaaatgtaca
     1321 cgaccataaa aacaagggca acgttgacta agaatttcat gattgtaaat aaaatgtaat
     1381 ttacagtata gtattttaat taatatacaa atgatttgat aataattctt atttaactat
     1441 aatatattgt gttgggttga attaaaggtc ccattctagg attaatctat aagatctagt
     1501 ccacaacgac cgatctgtgg tgcagtgtgt tatctatcat ggagataatt aaaatgataa
     1561 ccatctcgca aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc
     1621 tataaatatc tagataaaaa accgccacca tggtaggcgc tattgtttta tacgtgcttt
     1681 tggcggcggc ggtgccgcat tctgcctttg cgggatccct cctcgagctg cagcttaagc
     1741 ttactagtca tcatcaccac catcaccacc attagggccc gttattagta catttattaa
     1801 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca
     1861 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct
     1921 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt
     1981 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac
     2041 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt
     2101 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca
     2161 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaatagagc ttggacatat
     2221 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg
     2281 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg
     2341 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattgcgat cgcataactt
     2401 cgtatagcat acattatacg aagttataag ctcggaacgc tgcgctcggt cgttcggctg
     2461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2581 gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa aatcgacgct
     2641 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2701 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2761 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     2821 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2881 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2941 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3001 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3061 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3121 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3181 aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3241 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3301 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3361 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     3421 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     3481 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     3541 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     3601 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3661 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3721 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3781 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3841 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3901 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3961 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4021 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     4081 agcatttatc agggttattg tctcatgtcc gcgcgtt
//
