LOCUS       Exported                3856 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pQB4u
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 1, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        45..1011
                     /note="Lef2,603"
     promoter        1020..1129
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1136..1156
                     /note="L21"
     CDS             1157..1219
                     /codon_start=1
                     /note="BmNPV gp64sp"
                     /translation="MVGAIVLYVLLAAAVPHSAFA"
     misc_feature    1220..1261
                     /note="MCS"
     CDS             1262..1285
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     misc_feature    1296..1501
                     /note="p10 3'UTR"
     misc_signal     1520..2124
                     /note="1629"
     rep_origin      complement(2177..2925)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(2926..3794)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggg
     1021 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
     1081 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatct ctagaaactc
     1141 ctaaaaaacc gccaccatgg taggcgctat tgttttatac gtgcttttgg cggcggcggt
     1201 gccgcattct gcctttgcgg gatccctgca gaagcttgct agcctcgagg aattctccgg
     1261 agattacaag gatgacgatg acaagtaagc tgagcatgaa tcgtttttaa aataacaaat
     1321 caattgtttt ataatattcg tacgattctt tgattatgta ataaaatgtg atcattagga
     1381 agattacgaa aaatataaaa aatatgagtt ctgtgtgtat aacaaatgct gtaaacgcca
     1441 caattgtgtt tgttgcaaat aaacccatga ttatttgatt aaaattgttg ttttctttgt
     1501 tgcatgcaag ctgatccggg ttattagtac atttattaag cgctagattc tgtgcgttgt
     1561 tgatttacag acaattgttg tacgtatttt aataattcat taaatttata atctttaggg
     1621 tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa tttaaatatt
     1681 aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg ttgtttttcc
     1741 gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact tgccaaatct
     1801 tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg
     1861 acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt agtgtacaat
     1921 tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg cgtgttagct
     1981 ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac
     2041 gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa
     2101 tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata cattatacga
     2161 agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2221 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2281 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2341 aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2521 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2701 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2761 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2821 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2881 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgttaccaa
     2941 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3001 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3061 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3121 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3181 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3241 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3301 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3361 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3421 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3481 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3541 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3601 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3661 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3721 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3781 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3841 ctcatgtccg cgcgtt
//
