LOCUS       Exported                3832 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pQB4s
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 1, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        45..1011
                     /note="Lef2,603"
     promoter        1020..1129
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1136..1156
                     /note="L21"
     CDS             1157..1219
                     /codon_start=1
                     /note="BmNPV gp64sp"
                     /translation="MVGAIVLYVLLAAAVPHSAFA"
     misc_feature    1220..1261
                     /note="MCS"
     misc_feature    1272..1477
                     /note="p10 3'UTR"
     misc_signal     1496..2100
                     /note="1629"
     rep_origin      complement(2153..2901)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(2902..3770)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggg
     1021 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
     1081 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatct ctagaaactc
     1141 ctaaaaaacc gccaccatgg taggcgctat tgttttatac gtgcttttgg cggcggcggt
     1201 gccgcattct gcctttgcgg gatccctgca gaagcttgct agcctcgagg aattctccgg
     1261 ataagctgag catgaatcgt ttttaaaata acaaatcaat tgttttataa tattcgtacg
     1321 attctttgat tatgtaataa aatgtgatca ttaggaagat tacgaaaaat ataaaaaata
     1381 tgagttctgt gtgtataaca aatgctgtaa acgccacaat tgtgtttgtt gcaaataaac
     1441 ccatgattat ttgattaaaa ttgttgtttt ctttgttgca tgcaagctga tccgggttat
     1501 tagtacattt attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg
     1561 tattttaata attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa
     1621 tgattttcag cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat
     1681 aggtttcgat tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat
     1741 ggattttcgc tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg
     1801 atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct
     1861 tttgtatttc tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat
     1921 agagcttgga catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg
     1981 tcccaaccct cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc
     2041 ctattaattg tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt
     2101 gcgatcgcat aacttcgtat agcatacatt atacgaagtt ataagctcgg aacgctgcgc
     2161 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2221 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2281 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgccccct gacgagcatc
     2341 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2401 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2461 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     2521 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2581 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2641 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2701 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2761 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2821 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2881 gaaaaaaagg atctcaagaa gatcctttgt taccaatgct taatcagtga ggcacctatc
     2941 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3001 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3061 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3121 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3181 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3241 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3301 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3361 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3421 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3481 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3541 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3601 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3661 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3721 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3781 tttcaatatt attgaagcat ttatcagggt tattgtctca tgtccgcgcg tt
//
