LOCUS       Exported                4438 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pQB4j
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 1, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        45..1011
                     /note="Lef2,603"
     promoter        1020..1129
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1136..1156
                     /note="L21"
     CDS             1157..1159
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             1163..1813
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /note="GST"
                     /translation="SPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKF
                     ELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYG
                     VSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLY
                     MDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             1814..1831
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /note="thrombin site"
                     /translation="LVPRGS"
     misc_feature    1826..1867
                     /note="MCS"
     misc_feature    1878..2083
                     /note="p10 3'UTR"
     misc_signal     2102..2706
                     /note="1629"
     rep_origin      complement(2759..3507)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(3508..4376)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggg
     1021 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
     1081 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatct ctagaaactc
     1141 ctaaaaaacc gccaccatgg tctcccctat actaggttat tggaaaatta agggccttgt
     1201 gcaacccact cgacttcttt tggaatatct tgaagaaaaa tatgaagagc atttgtatga
     1261 gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa ttgggtttgg agtttcccaa
     1321 tcttccttat tatattgatg gtgatgttaa attaacacag tctatggcca tcatacgtta
     1381 tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa gagcgtgcag agatttcaat
     1441 gcttgaagga gcggttttgg atattagata cggtgtttcg agaattgcat atagtaaaga
     1501 ctttgaaact ctcaaagttg attttcttag caagctacct gaaatgctga aaatgttcga
     1561 agatcgttta tgtcataaaa catatttaaa tggtgatcat gtaacccatc ctgacttcat
     1621 gttgtatgac gctcttgatg ttgttttata catggaccca atgtgcctgg atgcgttccc
     1681 aaaattagtt tgttttaaaa aacgtattga agctatccca caaattgata agtacttgaa
     1741 atccagcaag tatatagcat ggcctttgca gggctggcaa gccacgtttg gtggtggcga
     1801 ccatcctcca aaacttgtgc ctcgcggatc cctgcagaag cttgctagcc tcgaggaatt
     1861 ctccggataa gctgagcatg aatcgttttt aaaataacaa atcaattgtt ttataatatt
     1921 cgtacgattc tttgattatg taataaaatg tgatcattag gaagattacg aaaaatataa
     1981 aaaatatgag ttctgtgtgt ataacaaatg ctgtaaacgc cacaattgtg tttgttgcaa
     2041 ataaacccat gattatttga ttaaaattgt tgttttcttt gttgcatgca agctgatccg
     2101 ggttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt
     2161 tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa
     2221 atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg
     2281 taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta
     2341 tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct
     2401 ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta
     2461 tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg
     2521 ttaaatagag cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg
     2581 tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca
     2641 cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt
     2701 ggaattgcga tcgcataact tcgtatagca tacattatac gaagttataa gctcggaacg
     2761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg ccccctgacg
     2941 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3001 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3061 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     3121 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3181 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3241 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3301 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3361 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3421 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3481 cgcgcagaaa aaaaggatct caagaagatc ctttgttacc aatgcttaat cagtgaggca
     3541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3601 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3661 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3721 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3781 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3841 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3901 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3961 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4021 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4081 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4141 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4201 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4261 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4321 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4381 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgtc cgcgcgtt
//
