LOCUS       Exported                3802 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pQB4h
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 1, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        45..1011
                     /note="Lef2,603"
     promoter        1020..1129
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1136..1156
                     /note="L21"
     misc_feature    1155..1201
                     /note="MCS"
     CDS             1157..1159
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             1202..1231
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     misc_feature    1242..1447
                     /note="p10 3'UTR"
     misc_signal     1466..2070
                     /note="1629"
     rep_origin      complement(2123..2871)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(2872..3740)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggg
     1021 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
     1081 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatct ctagaaactc
     1141 ctaaaaaacc gccaccatgg gatccctgca gaagcttgct agcctcgagg aattctccgg
     1201 agaacagaag ctcatctcag aagaggatct gtaagctgag catgaatcgt ttttaaaata
     1261 acaaatcaat tgttttataa tattcgtacg attctttgat tatgtaataa aatgtgatca
     1321 ttaggaagat tacgaaaaat ataaaaaata tgagttctgt gtgtataaca aatgctgtaa
     1381 acgccacaat tgtgtttgtt gcaaataaac ccatgattat ttgattaaaa ttgttgtttt
     1441 ctttgttgca tgcaagctga tccgggttat tagtacattt attaagcgct agattctgtg
     1501 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct
     1561 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta
     1621 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt
     1681 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc
     1741 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa
     1801 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg
     1861 tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac atcgggcgtg
     1921 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc
     1981 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg
     2041 atcaaatttg tagttgagct ttttggaatt gcgatcgcat aacttcgtat agcatacatt
     2101 atacgaagtt ataagctcgg aacgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2161 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2221 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     2281 ttccataggc tccgccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2461 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2701 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttgt
     2881 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2941 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3001 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3061 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3121 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3181 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3241 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3301 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3361 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3421 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3481 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3541 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3601 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3661 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3721 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3781 tattgtctca tgtccgcgcg tt
//
