LOCUS       Exported                3799 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pQB4f
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 1, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        45..1011
                     /note="Lef2,603"
     promoter        1020..1129
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1136..1156
                     /note="L21"
     misc_feature    1155..1201
                     /note="MCS"
     CDS             1157..1159
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             1202..1228
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    1239..1444
                     /note="p10 3'UTR"
     misc_signal     1463..2067
                     /note="1629"
     rep_origin      complement(2120..2868)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(2869..3737)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggg
     1021 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
     1081 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatct ctagaaactc
     1141 ctaaaaaacc gccaccatgg gatccctgca gaagcttgct agcctcgagg aattctccgg
     1201 atatccgtat gatgtaccgg attatgcata agctgagcat gaatcgtttt taaaataaca
     1261 aatcaattgt tttataatat tcgtacgatt ctttgattat gtaataaaat gtgatcatta
     1321 ggaagattac gaaaaatata aaaaatatga gttctgtgtg tataacaaat gctgtaaacg
     1381 ccacaattgt gtttgttgca aataaaccca tgattatttg attaaaattg ttgttttctt
     1441 tgttgcatgc aagctgatcc gggttattag tacatttatt aagcgctaga ttctgtgcgt
     1501 tgttgattta cagacaattg ttgtacgtat tttaataatt cattaaattt ataatcttta
     1561 gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt ctttatatct gaatttaaat
     1621 attaaatcct caatagattt gtaaaatagg tttcgattag tttcaaacaa gggttgtttt
     1681 tccgaaccga tggctggact atctaatgga ttttcgctca acgccacaaa acttgccaaa
     1741 tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg taataaaggt
     1801 tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt catcactgtc gttagtgtac
     1861 aattgactcg acgtaaacac gttaaataga gcttggacat atttaacatc gggcgtgtta
     1921 gctttattag gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt tgcttccgaa
     1981 gacgattttg ccatagccac acgacgccta ttaattgtgt cggctaacac gtccgcgatc
     2041 aaatttgtag ttgagctttt tggaattgcg atcgcataac ttcgtatagc atacattata
     2101 cgaagttata agctcggaac gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2161 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2221 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2281 cataggctcc gccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2461 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2701 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2761 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2821 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgttac
     2881 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2941 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3001 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3061 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     3121 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3181 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3241 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3301 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3361 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3421 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3481 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3541 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3601 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3661 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3721 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3781 tgtctcatgt ccgcgcgtt
//
