LOCUS       Exported                3796 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pQB4d
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 1, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        45..1011
                     /note="Lef2,603"
     promoter        1020..1129
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1136..1156
                     /note="L21"
     misc_feature    1155..1201
                     /note="MCS"
     CDS             1157..1159
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             1202..1225
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /note="FLAG"
                     /translation="DYKDDDDK"
     misc_feature    1236..1441
                     /note="p10 3'UTR"
     misc_signal     1460..2064
                     /note="1629"
     rep_origin      complement(2117..2865)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(2866..3734)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggg
     1021 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
     1081 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatct ctagaaactc
     1141 ctaaaaaacc gccaccatgg gatccctgca gaagcttgct agcctcgagg aattctccgg
     1201 agattacaag gatgacgatg acaagtaagc tgagcatgaa tcgtttttaa aataacaaat
     1261 caattgtttt ataatattcg tacgattctt tgattatgta ataaaatgtg atcattagga
     1321 agattacgaa aaatataaaa aatatgagtt ctgtgtgtat aacaaatgct gtaaacgcca
     1381 caattgtgtt tgttgcaaat aaacccatga ttatttgatt aaaattgttg ttttctttgt
     1441 tgcatgcaag ctgatccggg ttattagtac atttattaag cgctagattc tgtgcgttgt
     1501 tgatttacag acaattgttg tacgtatttt aataattcat taaatttata atctttaggg
     1561 tggtatgtta gagcgaaaat caaatgattt tcagcgtctt tatatctgaa tttaaatatt
     1621 aaatcctcaa tagatttgta aaataggttt cgattagttt caaacaaggg ttgtttttcc
     1681 gaaccgatgg ctggactatc taatggattt tcgctcaacg ccacaaaact tgccaaatct
     1741 tgtagcagca atctagcttt gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg
     1801 acgtcgttca aaatattatg cgcttttgta tttctttcat cactgtcgtt agtgtacaat
     1861 tgactcgacg taaacacgtt aaatagagct tggacatatt taacatcggg cgtgttagct
     1921 ttattaggcc gattatcgtc gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac
     1981 gattttgcca tagccacacg acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa
     2041 tttgtagttg agctttttgg aattgcgatc gcataacttc gtatagcata cattatacga
     2101 agttataagc tcggaacgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2161 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2221 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2281 aggctccgcc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2461 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgttaccaa
     2881 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2941 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3001 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3061 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3121 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3181 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3241 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3301 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3361 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3421 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3481 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3541 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3601 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3661 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3721 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3781 ctcatgtccg cgcgtt
//
