LOCUS       Exported                3805 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pQB4a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 1, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        45..1011
                     /note="Lef2,603"
     promoter        1020..1129
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1136..1156
                     /note="L21"
     CDS             1157..1159
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             1163..1180
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     misc_feature    1178..1234
                     /note="MCS"
     CDS             1181..1198
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /note="thrombin site"
                     /translation="LVPRGS"
     misc_feature    1245..1450
                     /note="p10 3'UTR"
     misc_signal     1469..2073
                     /note="1629"
     rep_origin      complement(2126..2874)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(2875..3743)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggg
     1021 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
     1081 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatct ctagaaactc
     1141 ctaaaaaacc gccaccatgg tccatcatca ccaccatcac cttgtgcctc gcggatccct
     1201 gcagaagctt gctagcctcg aggaattctc cggataagct gagcatgaat cgtttttaaa
     1261 ataacaaatc aattgtttta taatattcgt acgattcttt gattatgtaa taaaatgtga
     1321 tcattaggaa gattacgaaa aatataaaaa atatgagttc tgtgtgtata acaaatgctg
     1381 taaacgccac aattgtgttt gttgcaaata aacccatgat tatttgatta aaattgttgt
     1441 tttctttgtt gcatgcaagc tgatccgggt tattagtaca tttattaagc gctagattct
     1501 gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa
     1561 tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat
     1621 ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt
     1681 tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt
     1741 gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat
     1801 aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta
     1861 gtgtacaatt gactcgacgt aaacacgtta aatagagctt ggacatattt aacatcgggc
     1921 gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct
     1981 tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc
     2041 gcgatcaaat ttgtagttga gctttttgga attgcgatcg cataacttcg tatagcatac
     2101 attatacgaa gttataagct cggaacgctg cgctcggtcg ttcggctgcg gcgagcggta
     2161 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     2221 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     2281 tttttccata ggctccgccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2461 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2701 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2881 tgttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3781 ggttattgtc tcatgtccgc gcgtt
//
