LOCUS       Exported                3772 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pQB4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Bacmid Ltd.
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, August 1, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        45..1011
                     /note="Lef2,603"
     promoter        1020..1129
                     /note="p10 promoter"
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    1136..1156
                     /note="L21"
     misc_feature    1155..1201
                     /note="MCS"
     CDS             1157..1159
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     misc_feature    1212..1417
                     /note="p10 3'UTR"
     misc_signal     1436..2040
                     /note="1629"
     rep_origin      complement(2093..2841)
                     /direction=LEFT
                     /note="COLE1_ORI"
     CDS             complement(2842..3710)
                     /note="AmpR"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccgcggg
     1021 acctttaatt caacccaaca caatatatta tagttaaata agaattatta tcaaatcatt
     1081 tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatct ctagaaactc
     1141 ctaaaaaacc gccaccatgg gatccctgca gaagcttgct agcctcgagg aattctccgg
     1201 ataagctgag catgaatcgt ttttaaaata acaaatcaat tgttttataa tattcgtacg
     1261 attctttgat tatgtaataa aatgtgatca ttaggaagat tacgaaaaat ataaaaaata
     1321 tgagttctgt gtgtataaca aatgctgtaa acgccacaat tgtgtttgtt gcaaataaac
     1381 ccatgattat ttgattaaaa ttgttgtttt ctttgttgca tgcaagctga tccgggttat
     1441 tagtacattt attaagcgct agattctgtg cgttgttgat ttacagacaa ttgttgtacg
     1501 tattttaata attcattaaa tttataatct ttagggtggt atgttagagc gaaaatcaaa
     1561 tgattttcag cgtctttata tctgaattta aatattaaat cctcaataga tttgtaaaat
     1621 aggtttcgat tagtttcaaa caagggttgt ttttccgaac cgatggctgg actatctaat
     1681 ggattttcgc tcaacgccac aaaacttgcc aaatcttgta gcagcaatct agctttgtcg
     1741 atattcgttt gtgttttgtt ttgtaataaa ggttcgacgt cgttcaaaat attatgcgct
     1801 tttgtatttc tttcatcact gtcgttagtg tacaattgac tcgacgtaaa cacgttaaat
     1861 agagcttgga catatttaac atcgggcgtg ttagctttat taggccgatt atcgtcgtcg
     1921 tcccaaccct cgtcgttaga agttgcttcc gaagacgatt ttgccatagc cacacgacgc
     1981 ctattaattg tgtcggctaa cacgtccgcg atcaaatttg tagttgagct ttttggaatt
     2041 gcgatcgcat aacttcgtat agcatacatt atacgaagtt ataagctcgg aacgctgcgc
     2101 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2161 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgccccct gacgagcatc
     2281 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2341 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2401 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     2461 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2521 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2581 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2641 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2701 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2761 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2821 gaaaaaaagg atctcaagaa gatcctttgt taccaatgct taatcagtga ggcacctatc
     2881 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2941 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3001 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3061 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3121 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3181 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3241 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3301 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3361 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3421 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3481 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3541 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3601 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3661 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3721 tttcaatatt attgaagcat ttatcagggt tattgtctca tgtccgcgcg tt
//
