LOCUS Exported 5394 bp ds-DNA circular SYN 22-JAN-2019 DEFINITION Baculovirus transfer plasmid with the p6.9 and polh tandem promoters, encoding a gp64 signal peptide, an N-terminal 6xHis and a C-terminal 8xHis cassette. ACCESSION . VERSION . KEYWORDS pQB3s SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5394) AUTHORS Bacmid Ltd. TITLE Direct Submission JOURNAL Exported Tuesday, January 22, 2019 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5394 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1157..1226 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1157..1167 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1227..1340 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1341..1403 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1341..1403 /organism="synthetic DNA construct" /mol_type="other DNA" source 1341..1400 /organism="synthetic DNA construct" /mol_type="other DNA" source 1341..1400 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1341..1400 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1341..1400 /organism="synthetic DNA construct" /mol_type="other DNA" source 1341..1400 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1404..1421 /organism="synthetic DNA construct" /mol_type="other DNA" source 1404..1421 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1404..1409 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1404..1409 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1404..1409 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1404..1409 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1410..1415 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1410..1415 /organism="synthetic DNA construct" /mol_type="other DNA" source 1410..1415 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1428..1433 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1428..1430 /organism="synthetic DNA construct" /mol_type="other DNA" source 1428..1430 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1428..1430 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1434..1439 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" misc_recomb 329..1156 /note="baculovirus recombination region (lef2/ORF603)" /note="contains ORF603 and part of lef2" CDS 329..453 /codon_start=3 /product="baculovirus late expression factor 2" /note="lef2" /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(491..1096) /codon_start=1 /product="baculovirus ORF603 protein" /note="ORF603" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 1168..1226 /note="p6.9" misc_feature 1209 /note="TSS" promoter 1227..1318 /note="polh promoter" misc_feature 1319 /note="+1" CDS 1341..1403 /codon_start=1 /note="gp64 signalP" /translation="MVSAIVLYVLLAAAAHSAFAA" misc_feature 1404..1445 /note="MCS" CDS 1446..1469 /codon_start=1 /note="8xHis" /translation="HHHHHHHH" misc_recomb 1498..2855 /note="baculovirus recombination region (ORF1629)" /note="contains part of ORF1629" CDS complement(1514..2855) /codon_start=2 /product="baculovirus capsid-associated protein" /note="ORF1629" /note="required for viral replication" /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" rep_origin complement(3118..3706) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3877..4737) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4738..4842) /gene="bla" /note="AmpR promoter" rep_origin 4869..5324 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 1141 tcaatatata gttgctgatc agatcccggg agttcagtcg tcgaatgcaa aacgtaaaaa 1201 atattaataa ggtaaaaact acagctatca tggagataat taaaatgata accatctcgc 1261 aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat 1321 ctagataaaa aaccgccacc atggtaagcg ctattgtttt atatgtgctt ttggcggcgg 1381 cggcgcattc tgcctttgcg gccggatccc tgcagaagct tctcgaggaa ttcgctagct 1441 ccggacacca ccatcaccat caccatcact aagctgagcg catgcaagct gatccgggtt 1501 attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac aattgttgta 1561 cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga gcgaaaatca 1621 aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata gatttgtaaa 1681 ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct ggactatcta 1741 atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat ctagctttgt 1801 cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa atattatgcg 1861 cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta aacacgttaa 1921 atagagcttg gacatattta acatcgggcg tgttagcttt attaggccga ttatcgtcgt 1981 cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata gccacacgac 2041 gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag ctttttggaa 2101 ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc gattttaatt 2161 cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac ggcaaatcta 2221 ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc gcaggcgggg 2281 ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt ttaggctcaa 2341 atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg ggcgccgttt 2401 ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct tccaacaatt 2461 gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt ggagcgggcg 2521 gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta ggcacgggag 2581 aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt tcgcgcacga 2641 ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg cttcgaggca 2701 gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa aaatctgcta 2761 taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc tcaatgtaag 2821 caattgtatt gtaaagagat tgtctcaagc tcggatcgat cccgcacgcc gataacaagc 2881 cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc gaggtttaaa 2941 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 3001 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 3061 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 3121 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 3181 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 3241 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 3301 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 3361 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 3421 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 3481 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 3541 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 3601 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 3661 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 3721 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 3781 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 3841 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 3901 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 3961 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 4021 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 4081 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 4141 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 4201 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 4261 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 4321 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 4381 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 4441 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 4501 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 4561 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 4621 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 4681 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 4741 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 4801 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 4861 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 4921 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 4981 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 5041 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 5101 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 5161 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 5221 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 5281 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt cgccattcag 5341 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gcca //