LOCUS       Exported                3956 bp ds-DNA     circular SYN 26-APR-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pQB3a
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   yanyu
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, December 31, 2024 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1028..1517
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     source          1028..1086
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     source          1087..1200
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1201..1248
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1231..1248
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1231..1236
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1231..1236
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1231..1236
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1231..1236
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1237..1242
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     source          1237..1242
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1237..1242
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1255..1260
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     source          1255..1257
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1255..1257
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1255..1257
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /mol_type="genomic DNA"
                     /clone="C6"
                     /db_xref="taxon:46015"
     source          1261..1266
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     source          1307..1495
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /host="Autographa californica"
                     /strain="E2"
                     /mol_type="genomic DNA"
                     /country="USA"
                     /db_xref="taxon:46015"
     source          1496..1512
                     /organism="Autographa californica nucleopolyhedrovirus"
                     /host="Autographa californica"
                     /strain="E2"
                     /mol_type="genomic DNA"
                     /country="USA"
                     /db_xref="taxon:46015"
     enhancer        45..1011
                     /label=Lef2,603
                     /note="Lef2,603"
     promoter        1028..1086
                     /note="p6.9"
     misc_feature    1069
                     /note="TSS"
     promoter        1087..1178
                     /note="polh promoter"
     misc_feature    1179
                     /note="+1"
     CDS             1201..1203
                     /codon_start=1
                     /note="ATG"
                     /translation="M"
     CDS             1207..1224
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     misc_feature    1226..1272
                     /note="MCS"
     CDS             1273..1296
                     /codon_start=1
                     /note="8xHis"
                     /translation="HHHHHHHH"
     misc_feature    1307..1512
                     /note="p10 3'UTR"
     gene            complement(1318..1495)
                     /gene="Ac-p74-pif"
                     /note="Ac-p74-pif"
     polyA_signal    1371..1376
                     /note="1st aauaaa"
     polyA_signal    1469..1474
                     /note="2nd aauaaa"
     gene            complement(1496..1512)
                     /gene="Ac-p74-pif"
                     /note="Ac-p74-pif"
     misc_signal     1519..2224
                     /label=1629
                     /note="1629"
     oriT            2433..3021
                     /note="ColEI ori"
     CDS             complement(3034..3894)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggtacctac
     1021 tgatatcggg agttcagtcg tcgaatgcaa aacgtaaaaa atattaataa ggtaaaaact
     1081 acagctatca tggagataat taaaatgata accatctcgc aaataaataa gtattttact
     1141 gttttcgtaa cagttttgta ataaaaaaac ctataaatat ctagataaaa aaccgccacc
     1201 atggtccatc atcaccacca tcactccgcg ggatccctgc agaagcttct cgaggaattc
     1261 gctagctccg gacaccacca tcaccatcac catcactaag ctgagcatga atcgttttta
     1321 aaataacaaa tcaattgttt tataatattc gtacgattct ttgattatgt aataaaatgt
     1381 gatcattagg aagattacga aaaatataaa aaatatgagt tctgtgtgta taacaaatgc
     1441 tgtaaacgcc acaattgtgt ttgttgcaaa taaacccatg attatttgat taaaattgtt
     1501 gttttctttg ttgcatgcgg aggaaattct ccttgaagtt tccctggtgt tcaaagtaaa
     1561 ggagtttgca ccagacgcac ctctgttcac tggtccggcg tattaaaaca cgatacattg
     1621 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg
     1681 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat
     1741 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta
     1801 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc
     1861 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt
     1921 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg
     1981 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt
     2041 aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc
     2101 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg
     2161 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg
     2221 aattgcgatc gcataacttc gtatagcata cattatacga agttataagc tcggaacgct
     2281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc ccctgacgag
     2461 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2521 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2581 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     2641 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2701 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2761 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2821 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2881 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2941 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3001 cgcagaaaaa aaggatctca agaagatcct ttgttaccaa tgcttaatca gtgaggcacc
     3061 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgtccg cgcgtt
//
