LOCUS Exported 5305 bp ds-DNA circular SYN 22-JAN-2019 DEFINITION Baculovirus transfer plasmid with the polh promoter, encoding an N-terminal 6xHis and CN-terminal 8xHis cassette. ACCESSION . VERSION . KEYWORDS pQB2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5305) AUTHORS Bacmid Ltd. TITLE Direct Submission JOURNAL Exported Tuesday, January 22, 2019 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5305 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1157..1170 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1171..1284 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1285..1332 /organism="synthetic DNA construct" /mol_type="other DNA" source 1315..1332 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1315..1320 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1315..1320 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1315..1320 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1315..1320 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1321..1326 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1321..1326 /organism="synthetic DNA construct" /mol_type="other DNA" source 1321..1326 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1339..1344 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1339..1341 /organism="synthetic DNA construct" /mol_type="other DNA" source 1339..1341 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1339..1341 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1345..1350 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" misc_recomb 329..1156 /note="baculovirus recombination region (lef2/ORF603)" /note="contains ORF603 and part of lef2" CDS 329..453 /codon_start=3 /product="baculovirus late expression factor 2" /note="lef2" /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(491..1096) /codon_start=1 /product="baculovirus ORF603 protein" /note="ORF603" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 1171..1262 /note="polh promoter" misc_feature 1263 /note="+1" CDS 1285..1287 /codon_start=1 /note="ATG" /translation="M" CDS 1291..1308 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" misc_feature 1310..1356 /note="MCS" CDS 1357..1380 /codon_start=1 /note="8xHis" /translation="HHHHHHHH" misc_recomb 1409..2766 /note="baculovirus recombination region (ORF1629)" /note="contains part of ORF1629" CDS complement(1425..2766) /codon_start=2 /product="baculovirus capsid-associated protein" /note="ORF1629" /note="required for viral replication" /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" rep_origin complement(3029..3617) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3788..4648) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4649..4753) /gene="bla" /note="AmpR promoter" rep_origin 4780..5235 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 1141 tcaatatata gttgctgatc agatcccggg atcatggaga taattaaaat gataaccatc 1201 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa 1261 atatctagat aaaaaaccgc caccatggtc catcatcacc accatcactc cgcgggatcc 1321 ctgcagaagc ttctcgagga attcgctagc tccggacacc accatcacca tcaccatcac 1381 taagctgagc gcatgcaagc tgatccgggt tattagtaca tttattaagc gctagattct 1441 gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa 1501 tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat 1561 ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt 1621 tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt 1681 gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat 1741 aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta 1801 gtgtacaatt gactcgacgt aaacacgtta aatagagctt ggacatattt aacatcgggc 1861 gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct 1921 tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc 1981 gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg tttttgggcg 2041 ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag cgatggtgca 2101 ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg agctgatgat 2161 aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg cggaggtggt 2221 ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac agtcggcacc 2281 tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag acgagtgcga 2341 tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt 2401 tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg tggtggtggt 2461 ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc cgccggtata 2521 atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc 2581 tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta 2641 taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct atcgtttacc 2701 gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag 2761 ctcggatcga tcccgcacgc cgataacaag ccttttcatt tttactacag cattgtagtg 2821 gcgagacact tcgctgtcgt cgaggtttaa acgcttcctc gctcactgac tcgctgcgct 2881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 2941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 3001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 3661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 3721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 3781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 3841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 3901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 3961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 4021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 4081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 4141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 4201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 4261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 4321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 4381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 4441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat 4801 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag 4861 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc 4921 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc 4981 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt 5041 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa 5101 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg 5161 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat 5221 taacgtttac aatttcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 5281 tgcgggcctc ttcgctatta cgcca //