LOCUS Exported 5302 bp ds-DNA circular SYN 22-JAN-2019 DEFINITION Baculovirus transfer plasmid with the p6.9 promoter, encoding a gp64 signal peptide, an N-terminal 6xHis and a C-terminal 8xHis cassette. ACCESSION . VERSION . KEYWORDS pQB1s SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5302) AUTHORS Bacmid Ltd. TITLE Direct Submission JOURNAL Exported Tuesday, January 22, 2019 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5302 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1157..1167 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1168..1248 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1249..1311 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1249..1311 /organism="synthetic DNA construct" /mol_type="other DNA" source 1249..1308 /organism="synthetic DNA construct" /mol_type="other DNA" source 1249..1308 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1249..1308 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1249..1308 /organism="synthetic DNA construct" /mol_type="other DNA" source 1249..1308 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1312..1329 /organism="synthetic DNA construct" /mol_type="other DNA" source 1312..1329 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1312..1317 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1312..1317 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1312..1317 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1312..1317 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1318..1323 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1318..1323 /organism="synthetic DNA construct" /mol_type="other DNA" source 1318..1323 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1336..1341 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1336..1338 /organism="synthetic DNA construct" /mol_type="other DNA" source 1336..1338 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1336..1338 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1342..1347 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" misc_recomb 329..1156 /note="baculovirus recombination region (lef2/ORF603)" /note="contains ORF603 and part of lef2" CDS 329..453 /codon_start=3 /product="baculovirus late expression factor 2" /note="lef2" /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(491..1096) /codon_start=1 /product="baculovirus ORF603 protein" /note="ORF603" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 1168..1248 /note="p6.9 promoter" gene 1168..1170 /gene="Ac-p40" /gene_synonym="AcOrf-101" /note="Ac-p40" misc_feature 1209 /note="TSS" CDS 1249..1311 /codon_start=1 /note="gp64 signalP" /translation="MVSAIVLYVLLAAAAHSAFAA" misc_feature 1312..1353 /note="MCS" CDS 1354..1377 /codon_start=1 /note="8xHis" /translation="HHHHHHHH" misc_recomb 1406..2763 /note="baculovirus recombination region (ORF1629)" /note="contains part of ORF1629" CDS complement(1422..2763) /codon_start=2 /product="baculovirus capsid-associated protein" /note="ORF1629" /note="required for viral replication" /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" rep_origin complement(3026..3614) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3785..4645) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4646..4750) /gene="bla" /note="AmpR promoter" rep_origin 4777..5232 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 1141 tcaatatata gttgctgatc agatcccggg agttcagtcg tcgaatgcaa aacgtaaaaa 1201 atattaataa ggtaaaaact acagctacat aaattacaca atttaaccat ggtaagcgct 1261 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc cggatccctg 1321 cagaagcttc tcgaggaatt cgctagctcc ggacaccacc atcaccatca ccatcactaa 1381 gctgagcgca tgcaagctga tccgggttat tagtacattt attaagcgct agattctgtg 1441 cgttgttgat ttacagacaa ttgttgtacg tattttaata attcattaaa tttataatct 1501 ttagggtggt atgttagagc gaaaatcaaa tgattttcag cgtctttata tctgaattta 1561 aatattaaat cctcaataga tttgtaaaat aggtttcgat tagtttcaaa caagggttgt 1621 ttttccgaac cgatggctgg actatctaat ggattttcgc tcaacgccac aaaacttgcc 1681 aaatcttgta gcagcaatct agctttgtcg atattcgttt gtgttttgtt ttgtaataaa 1741 ggttcgacgt cgttcaaaat attatgcgct tttgtatttc tttcatcact gtcgttagtg 1801 tacaattgac tcgacgtaaa cacgttaaat agagcttgga catatttaac atcgggcgtg 1861 ttagctttat taggccgatt atcgtcgtcg tcccaaccct cgtcgttaga agttgcttcc 1921 gaagacgatt ttgccatagc cacacgacgc ctattaattg tgtcggctaa cacgtccgcg 1981 atcaaatttg tagttgagct ttttggaatt atttctgatt gcgggcgttt ttgggcgggt 2041 ttcaatctaa ctgtgcccga ttttaattca gacaacacgt tagaaagcga tggtgcaggc 2101 ggtggtaaca tttcagacgg caaatctact aatggcggcg gtggtggagc tgatgataaa 2161 tctaccatcg gtggaggcgc aggcggggct ggcggcggag gcggaggcgg aggtggtggc 2221 ggtgatgcag acggcggttt aggctcaaat gtctctttag gcaacacagt cggcacctca 2281 actattgtac tggtttcggg cgccgttttt ggtttgaccg gtctgagacg agtgcgattt 2341 ttttcgtttc taatagcttc caacaattgt tgtctgtcgt ctaaaggtgc agcgggttga 2401 ggttccgtcg gcattggtgg agcgggcggc aattcagaca tcgatggtgg tggtggtggt 2461 ggaggcgctg gaatgttagg cacgggagaa ggtggtggcg gcggtgccgc cggtataatt 2521 tgttctggtt tagtttgttc gcgcacgatt gtgggcaccg gcgcaggcgc cgctggctgc 2581 acaacggaag gtcgtctgct tcgaggcagc gcttggggtg gtggcaattc aatattataa 2641 ttggaataca aatcgtaaaa atctgctata agcattgtaa tttcgctatc gtttaccgtg 2701 ccgatattta acaaccgctc aatgtaagca attgtattgt aaagagattg tctcaagctc 2761 ggatcgatcc cgcacgccga taacaagcct tttcattttt actacagcat tgtagtggcg 2821 agacacttcg ctgtcgtcga ggtttaaacg cttcctcgct cactgactcg ctgcgctcgg 2881 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 2941 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 3001 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 3061 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 3121 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 3181 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 3241 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 3301 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 3361 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 3421 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 3481 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 3541 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 3601 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 3661 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 3721 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 3781 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 3841 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 3901 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 3961 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 4021 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 4081 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 4141 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 4201 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 4261 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 4321 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 4381 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 4441 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 4501 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 4561 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 4621 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 4681 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 4741 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa 4801 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 4861 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 4921 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 4981 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 5041 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 5101 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 5161 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 5221 cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 5281 gggcctcttc gctattacgc ca //