LOCUS Exported 5471 bp ds-DNA circular SYN 06-NOV-2018 DEFINITION Baculovirus transfer plasmid with the gp64 promoter, encoding an N-terminal 6xHis-thrombin-S-Tag-enterokinase cassette. ACCESSION . VERSION . KEYWORDS pQB1064 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5471) AUTHORS Bacmid Ltd. TITLE Direct Submission JOURNAL Exported Tuesday, November 6, 2018 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5471 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1449..1498 /organism="synthetic DNA construct" /mol_type="other DNA" source 1481..1498 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1481..1486 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1481..1486 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1481..1486 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1481..1486 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1487..1492 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1487..1492 /organism="synthetic DNA construct" /mol_type="other DNA" source 1487..1492 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1505..1510 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1505..1507 /organism="synthetic DNA construct" /mol_type="other DNA" source 1505..1507 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1505..1507 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1511..1516 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" misc_recomb 329..1156 /note="baculovirus recombination region (lef2/ORF603)" /note="contains ORF603 and part of lef2" CDS 329..453 /codon_start=3 /product="baculovirus late expression factor 2" /note="lef2" /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(491..1096) /codon_start=1 /product="baculovirus ORF603 protein" /note="ORF603" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 1166..1268 /note="p10 promoter" promoter 1270..1448 /note="gp64 promoter" CDS 1451..1453 /codon_start=1 /note="ATG" /translation="M" CDS 1457..1474 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" misc_feature 1476..1522 /note="MCS" CDS 1523..1546 /codon_start=1 /note="8xHis" /translation="HHHHHHHH" misc_recomb 1575..2932 /note="baculovirus recombination region (ORF1629)" /note="contains part of ORF1629" CDS complement(1591..2932) /codon_start=2 /product="baculovirus capsid-associated protein" /note="ORF1629" /note="required for viral replication" /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" rep_origin complement(3195..3783) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3954..4814) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4815..4919) /gene="bla" /note="AmpR promoter" rep_origin 4946..5401 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 1141 tcaatatata gttgctgatc agatcccggg acctttaatt caacccaaca caatatatta 1201 tagttaaata agaattatta tcaaatcatt tgtatattaa ttaaaatact atactgtaaa 1261 ttacatttta tcccgttttt ataacagcca gataaaaata atcttatcaa ttaagataaa 1321 aagataagat tattaatcta acaacgtgcc ttgtgtcacg taggccagat aacggtcggg 1381 tatataagac gcctcaacgc tactagtaaa tcagtcacac caaggcttca ataaggaaca 1441 cacaagcacc atggtccatc atcaccacca tcactccgcg ggatccctgc agaagcttct 1501 cgaggaattc gctagctccg gacaccacca tcaccatcac catcactaag ctgagcgcat 1561 gcaagctgat ccgggttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt 1621 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta 1681 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc 1741 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc 1801 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag 1861 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc 1921 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact 1981 cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt tagctttatt 2041 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt 2101 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt 2161 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac 2221 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat 2281 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg 2341 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga 2401 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact 2461 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct 2521 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg 2581 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg 2641 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt 2701 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg 2761 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa 2821 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa 2881 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg gatcgatccc 2941 gcacgccgat aacaagcctt ttcattttta ctacagcatt gtagtggcga gacacttcgc 3001 tgtcgtcgag gtttaaacgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 3061 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 3121 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 3181 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 3241 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 3301 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 3361 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 3421 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 3481 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 3541 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 3601 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 3661 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 3721 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 3781 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 3841 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 3901 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 3961 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 4021 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 4081 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 4141 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 4201 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 4261 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 4321 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 4381 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 4441 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 4501 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 4561 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 4621 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 4681 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 4741 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 4801 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 4861 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 4921 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 4981 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 5041 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 5101 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 5161 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 5221 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 5281 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 5341 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 5401 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 5461 ctattacgcc a //