LOCUS Exported 5269 bp ds-DNA circular SYN 22-JAN-2019 DEFINITION Baculovirus transfer plasmid with the p6.9 promoter, encoding an N-terminal 6xHis and CN-terminal 8xHis cassette. ACCESSION . VERSION . KEYWORDS pQB1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5269) AUTHORS Bacmid Ltd. TITLE Direct Submission JOURNAL Exported Tuesday, January 22, 2019 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5269 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1157..1167 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1168..1248 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1249..1296 /organism="synthetic DNA construct" /mol_type="other DNA" source 1279..1296 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1279..1284 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1279..1284 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1279..1284 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1279..1284 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1285..1290 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1285..1290 /organism="synthetic DNA construct" /mol_type="other DNA" source 1285..1290 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1303..1308 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1303..1305 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1303..1305 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1303..1305 /organism="synthetic DNA construct" /mol_type="other DNA" source 1309..1314 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" misc_recomb 329..1156 /note="baculovirus recombination region (lef2/ORF603)" /note="contains ORF603 and part of lef2" CDS 329..453 /codon_start=3 /product="baculovirus late expression factor 2" /note="lef2" /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(491..1096) /codon_start=1 /product="baculovirus ORF603 protein" /note="ORF603" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 1168..1248 /note="p6.9 promoter" gene 1168..1170 /gene="Ac-p40" /gene_synonym="AcOrf-101" /note="Ac-p40" misc_feature 1209 /note="TSS" CDS 1249..1251 /codon_start=1 /note="ATG" /translation="M" CDS 1255..1272 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" misc_feature 1274..1320 /note="MCS" CDS 1321..1344 /codon_start=1 /note="8xHis" /translation="HHHHHHHH" misc_recomb 1373..2730 /note="baculovirus recombination region (ORF1629)" /note="contains part of ORF1629" CDS complement(1389..2730) /codon_start=2 /product="baculovirus capsid-associated protein" /note="ORF1629" /note="required for viral replication" /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" rep_origin complement(2993..3581) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3752..4612) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4613..4717) /gene="bla" /note="AmpR promoter" rep_origin 4744..5199 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 1141 tcaatatata gttgctgatc agatcccggg agttcagtcg tcgaatgcaa aacgtaaaaa 1201 atattaataa ggtaaaaact acagctacat aaattacaca atttaaccat ggtccatcat 1261 caccaccatc actccgcggg atccctgcag aagcttctcg aggaattcgc tagctccgga 1321 caccaccatc accatcacca tcactaagct gagcgcatgc aagctgatcc gggttattag 1381 tacatttatt aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat 1441 tttaataatt cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga 1501 ttttcagcgt ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg 1561 tttcgattag tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga 1621 ttttcgctca acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata 1681 ttcgtttgtg ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt 1741 gtatttcttt catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataga 1801 gcttggacat atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc 1861 caaccctcgt cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta 1921 ttaattgtgt cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt 1981 tctgattgcg ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac 2041 aacacgttag aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat 2101 ggcggcggtg gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc 2161 ggcggaggcg gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc 2221 tctttaggca acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt 2281 ttgaccggtc tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt 2341 ctgtcgtcta aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat 2401 tcagacatcg atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt 2461 ggtggcggcg gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg 2521 ggcaccggcg caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct 2581 tggggtggtg gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc 2641 attgtaattt cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt 2701 gtattgtaaa gagattgtct caagctcgga tcgatcccgc acgccgataa caagcctttt 2761 catttttact acagcattgt agtggcgaga cacttcgctg tcgtcgaggt ttaaacgctt 2821 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 2881 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 2941 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 3001 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 3061 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 3121 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 3181 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 3241 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 3301 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 3361 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 3421 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 3481 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 3541 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 3601 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 3661 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 3721 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 3781 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 3841 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 3901 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 3961 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 4021 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 4081 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 4141 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 4201 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 4261 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 4321 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 4381 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 4441 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 4501 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 4561 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 4621 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 4681 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 4741 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 4801 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 4861 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 4921 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 4981 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 5041 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 5101 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 5161 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccattcgcca ttcaggctgc 5221 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgcca //