LOCUS Exported 5355 bp ds-DNA circular SYN 07-NOV-2018 DEFINITION Display antigen on the surface of baculovirus. It could be used as subunit vaccine and DNA vaccine. ACCESSION . VERSION . KEYWORDS pQBD SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5355) AUTHORS Bacmid Ltd. TITLE Direct Submission JOURNAL Exported Wednesday, November 7, 2018 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5355 /organism="synthetic DNA construct" /mol_type="other DNA" source 2333..2527 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 2396..2398 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 2423..2428 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 2423..2428 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 2423..2428 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 2423..2428 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 2435..2437 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" enhancer 45..1011 /label=Lef2,603 /note="Lef2,603" enhancer 1079..1443 /label=CMV Enhancer /note="CMV Enhancer" promoter 1449..1726 /label=Chicken B-Actin Promoter /note="Chicken B-Actin Promoter" exon 2082..2149 /label=Exon 3 (Rabbit Globin) /note="Exon 3" promoter 2150..2166 /label=T7 Promoter /note="T7 Promoter" protein_bind 2171..2191 /label=lac Operator /note="lac Operator" promoter 2205..2318 /label=p10 promoter + 5' UTR /note="p10 promoter + 5' UTR" misc_feature 2333..2395 /note="gp64 signalP" gene 2396..2398 /gene="Ac-gp64" /gene_synonym="AcOrf-128" /note="Ac-gp64" CDS 2399..2422 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /note="FLAG" /translation="DYKDDDDK" misc_feature 2423..2440 /note="MSC" misc_feature 2438..2506 /note="gp64_TM" misc_feature 2507..2527 /note="gp64 Inner Domain" CDS 2537..2560 /codon_start=1 /note="His-tag" /translation="HHHHHHHH" polyA_signal 2572..2854 /label=Rabbit B-Globin Poly A /note="Rabbit B-Globin Poly A" terminator 2858..2905 /label=T7_TERM /note="T7_TERM" misc_signal 2918..3623 /label=1629 /note="1629" rep_origin 3834 /label=COLE1_ORI /note="COLE1_ORI" CDS complement(4425..5293) /codon_start=1 /label=AmpR /note="AmpR" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW*QR" ORIGIN 1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga 61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat 121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca 181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa 241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt 301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa 361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag 421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt 481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt 541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt 601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg 661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc 721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt 781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt 841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc 901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg 961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg 1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct 1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc 1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg 1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg 1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag 1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg 1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact 1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta 1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct 1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc 1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc 2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc 2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat 2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatggtaag 2341 cgctattgtt ttatatgtgc ttttggcggc ggcggcgcat tctgcctttg cggctagcga 2401 ctacaaagac gatgacgaca agggatccaa gcttgaattc atgtttggtc atgtagttaa 2461 ctttgtaatt atattaattg tgattttatt tttgtactgt atgattagaa accgtaatag 2521 acaatattaa ctcgagcacc accatcacca tcaccatcac taagtgatta acctcaggtg 2581 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac 2641 tgagatcgat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca 2701 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt 2761 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta 2821 tttggtttag agtttggcaa catatgccca tatgtaacta gcataacccc ttggggcctc 2881 taaacgggtc ttgaggggtt ttttgctgaa agcatgcgga ggaaattctc cttgaagttt 2941 ccctggtgtt caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt 3001 attaaaacac gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt 3061 gatttacaga caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt 3121 ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta 3181 aatcctcaat agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg 3241 aaccgatggc tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt 3301 gtagcagcaa tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga 3361 cgtcgttcaa aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt 3421 gactcgacgt aaacacgtta aatagagctt ggacatattt aacatcgggc gtgttagctt 3481 tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg 3541 attttgccat agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat 3601 ttgtagttga gctttttgga attgcgatcg cataacttcg tatagcatac attatacgaa 3661 gttataagct cggaacgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 3721 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 3781 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 3841 ggctccgccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 3901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 3961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 4021 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 4081 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 4141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 4201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 4261 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 4321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 4381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgttaccaat 4441 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 4501 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 4561 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 4621 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 4681 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 4741 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 4801 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 4861 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 4921 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 4981 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 5041 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 5101 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 5161 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 5221 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 5281 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 5341 tcatgtccgc gcgtt //