LOCUS Exported 5361 bp ds-DNA circular SYN 22-JAN-2019 DEFINITION Baculovirus transfer plasmid with the p6.9 and polh tandem promoters, encoding an N-terminal 6xHis and C-terminal 8xHis cassette. ACCESSION . VERSION . KEYWORDS pQB3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5361) AUTHORS Bacmid Ltd. TITLE Direct Submission JOURNAL Exported Tuesday, January 22, 2019 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5361 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1157..1226 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1157..1167 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1227..1340 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1341..1388 /organism="synthetic DNA construct" /mol_type="other DNA" source 1371..1388 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1371..1376 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1371..1376 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1371..1376 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1371..1376 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1377..1382 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1377..1382 /organism="synthetic DNA construct" /mol_type="other DNA" source 1377..1382 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1395..1400 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1395..1397 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1395..1397 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1395..1397 /organism="synthetic DNA construct" /mol_type="other DNA" source 1401..1406 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" misc_recomb 329..1156 /note="baculovirus recombination region (lef2/ORF603)" /note="contains ORF603 and part of lef2" CDS 329..453 /codon_start=3 /product="baculovirus late expression factor 2" /note="lef2" /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(491..1096) /codon_start=1 /product="baculovirus ORF603 protein" /note="ORF603" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 1168..1226 /note="p6.9" misc_feature 1209 /note="TSS" promoter 1227..1318 /note="polh promoter" misc_feature 1319 /note="+1" CDS 1341..1343 /codon_start=1 /note="ATG" /translation="M" CDS 1347..1364 /codon_start=1 /product="6xHis affinity tag" /note="6xHis" /translation="HHHHHH" misc_feature 1366..1412 /note="MCS" CDS 1413..1436 /codon_start=1 /note="8xHis" /translation="HHHHHHHH" misc_recomb 1465..2822 /note="baculovirus recombination region (ORF1629)" /note="contains part of ORF1629" CDS complement(1481..2822) /codon_start=2 /product="baculovirus capsid-associated protein" /note="ORF1629" /note="required for viral replication" /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" rep_origin complement(3085..3673) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3844..4704) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4705..4809) /gene="bla" /note="AmpR promoter" rep_origin 4836..5291 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 1141 tcaatatata gttgctgatc agatcccggg agttcagtcg tcgaatgcaa aacgtaaaaa 1201 atattaataa ggtaaaaact acagctatca tggagataat taaaatgata accatctcgc 1261 aaataaataa gtattttact gttttcgtaa cagttttgta ataaaaaaac ctataaatat 1321 ctagataaaa aaccgccacc atggtccatc atcaccacca tcactccgcg ggatccctgc 1381 agaagcttct cgaggaattc gctagctccg gacaccacca tcaccatcac catcactaag 1441 ctgagcgcat gcaagctgat ccgggttatt agtacattta ttaagcgcta gattctgtgc 1501 gttgttgatt tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt 1561 tagggtggta tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa 1621 atattaaatc ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt 1681 tttccgaacc gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca 1741 aatcttgtag cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag 1801 gttcgacgtc gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt 1861 acaattgact cgacgtaaac acgttaaata gagcttggac atatttaaca tcgggcgtgt 1921 tagctttatt aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg 1981 aagacgattt tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga 2041 tcaaatttgt agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt 2101 tcaatctaac tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg 2161 gtggtaacat ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat 2221 ctaccatcgg tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg 2281 gtgatgcaga cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa 2341 ctattgtact ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt 2401 tttcgtttct aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag 2461 gttccgtcgg cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg 2521 gaggcgctgg aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt 2581 gttctggttt agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca 2641 caacggaagg tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat 2701 tggaatacaa atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc 2761 cgatatttaa caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg 2821 gatcgatccc gcacgccgat aacaagcctt ttcattttta ctacagcatt gtagtggcga 2881 gacacttcgc tgtcgtcgag gtttaaacgc ttcctcgctc actgactcgc tgcgctcggt 2941 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 3001 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 3061 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 3121 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 3181 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3241 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 3301 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 3361 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 3421 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 3481 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 3541 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 3601 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 3661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 3721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 3781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 3841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 3901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 3961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 4021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 4081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 4141 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 4201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 4261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 4321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 4381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 4441 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 4501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 4561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 4621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 4681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 4741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 4801 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag 4861 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 4921 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc 4981 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa 5041 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg 5101 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac 5161 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta 5221 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac 5281 gtttacaatt tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 5341 ggcctcttcg ctattacgcc a //