LOCUS Exported 5338 bp ds-DNA circular SYN 22-JAN-2019 DEFINITION Baculovirus transfer plasmid with the polh promoter, encoding a gp64 signal peptide, an N-terminal 6xHis and a C-terminal 8xHis cassette. ACCESSION . VERSION . KEYWORDS pQB2s SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5338) AUTHORS Bacmid Ltd. TITLE Direct Submission JOURNAL Exported Tuesday, January 22, 2019 from SnapGene 3.2.1 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5338 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1157..1170 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1171..1284 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1285..1347 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1285..1347 /organism="synthetic DNA construct" /mol_type="other DNA" source 1285..1344 /organism="synthetic DNA construct" /mol_type="other DNA" source 1285..1344 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1285..1344 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1285..1344 /organism="synthetic DNA construct" /mol_type="other DNA" source 1285..1344 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1348..1365 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1348..1365 /organism="synthetic DNA construct" /mol_type="other DNA" source 1348..1353 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1348..1353 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1348..1353 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1348..1353 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1354..1359 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1354..1359 /organism="synthetic DNA construct" /mol_type="other DNA" source 1354..1359 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1372..1377 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" source 1372..1374 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1372..1374 /organism="Autographa californica nucleopolyhedrovirus" /mol_type="genomic DNA" /clone="C6" /db_xref="taxon:46015" source 1372..1374 /organism="synthetic DNA construct" /mol_type="other DNA" source 1378..1383 /organism="synthetic DNA construct" /lab_host="Insect Cells" /mol_type="other DNA" misc_recomb 329..1156 /note="baculovirus recombination region (lef2/ORF603)" /note="contains ORF603 and part of lef2" CDS 329..453 /codon_start=3 /product="baculovirus late expression factor 2" /note="lef2" /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY" CDS complement(491..1096) /codon_start=1 /product="baculovirus ORF603 protein" /note="ORF603" /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR" promoter 1171..1262 /note="polh promoter" misc_feature 1263 /note="+1" CDS 1285..1347 /codon_start=1 /note="gp64 signalP" /translation="MVSAIVLYVLLAAAAHSAFAA" misc_feature 1348..1389 /note="MCS" CDS 1390..1413 /codon_start=1 /note="8xHis" /translation="HHHHHHHH" misc_recomb 1442..2799 /note="baculovirus recombination region (ORF1629)" /note="contains part of ORF1629" CDS complement(1458..2799) /codon_start=2 /product="baculovirus capsid-associated protein" /note="ORF1629" /note="required for viral replication" /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA" rep_origin complement(3062..3650) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3821..4681) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4682..4786) /gene="bla" /note="AmpR promoter" rep_origin 4813..5268 /direction=RIGHT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" ORIGIN 1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 1141 tcaatatata gttgctgatc agatcccggg atcatggaga taattaaaat gataaccatc 1201 tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa 1261 atatctagat aaaaaaccgc caccatggta agcgctattg ttttatatgt gcttttggcg 1321 gcggcggcgc attctgcctt tgcggccgga tccctgcaga agcttctcga ggaattcgct 1381 agctccggac accaccatca ccatcaccat cactaagctg agcgcatgca agctgatccg 1441 ggttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac agacaattgt 1501 tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt tagagcgaaa 1561 atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc aatagatttg 1621 taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat ggctggacta 1681 tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag caatctagct 1741 ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt caaaatatta 1801 tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga cgtaaacacg 1861 ttaaatagag cttggacata tttaacatcg ggcgtgttag ctttattagg ccgattatcg 1921 tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc catagccaca 1981 cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt tgagcttttt 2041 ggaattattt ctgattgcgg gcgtttttgg gcgggtttca atctaactgt gcccgatttt 2101 aattcagaca acacgttaga aagcgatggt gcaggcggtg gtaacatttc agacggcaaa 2161 tctactaatg gcggcggtgg tggagctgat gataaatcta ccatcggtgg aggcgcaggc 2221 ggggctggcg gcggaggcgg aggcggaggt ggtggcggtg atgcagacgg cggtttaggc 2281 tcaaatgtct ctttaggcaa cacagtcggc acctcaacta ttgtactggt ttcgggcgcc 2341 gtttttggtt tgaccggtct gagacgagtg cgattttttt cgtttctaat agcttccaac 2401 aattgttgtc tgtcgtctaa aggtgcagcg ggttgaggtt ccgtcggcat tggtggagcg 2461 ggcggcaatt cagacatcga tggtggtggt ggtggtggag gcgctggaat gttaggcacg 2521 ggagaaggtg gtggcggcgg tgccgccggt ataatttgtt ctggtttagt ttgttcgcgc 2581 acgattgtgg gcaccggcgc aggcgccgct ggctgcacaa cggaaggtcg tctgcttcga 2641 ggcagcgctt ggggtggtgg caattcaata ttataattgg aatacaaatc gtaaaaatct 2701 gctataagca ttgtaatttc gctatcgttt accgtgccga tatttaacaa ccgctcaatg 2761 taagcaattg tattgtaaag agattgtctc aagctcggat cgatcccgca cgccgataac 2821 aagccttttc atttttacta cagcattgta gtggcgagac acttcgctgt cgtcgaggtt 2881 taaacgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 2941 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 3001 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 3061 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 3121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 3181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 3241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 3301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 3361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 3421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 3481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 3541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 3601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 3661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 3721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 3781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 3841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 3901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 3961 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 4141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 4201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 4261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 4321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 4381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 4441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 4501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 4561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 4621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 4681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 4741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 4801 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 4861 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 4921 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 4981 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 5041 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 5101 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 5161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 5221 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc cattcgccat 5281 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgcca //