LOCUS       pBac5        5517 bp    DNA   circular            25-SEP-2002
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.informaxinc.com/
COMMENT     VNTDATE|276181381|
COMMENT     VNTDBDATE|278699069|
COMMENT     VNTNAME|pBac5|
COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     promoter        1191..1367
                     /vntifkey="29"
                     /label=gp64\promoter
     5'UTR           1371..1371
                     /vntifkey="52"
                     /label=wt\gp64\5'UTR-1\position
     protein_bind    1378..1395
                     /vntifkey="31"
                     /label=his\tag
     protein_bind    1426..1470
                     /vntifkey="31"
                     /label=S\tag
     protein_bind    1567..1584
                     /vntifkey="31"
                     /label=his\tag
     protein_bind    1405..1422
                     /vntifkey="31"
                     /label=thrombin
     protein_bind    1486..1500
                     /vntifkey="31"
                     /label=enterokinase
     CDS             complement(4003..4860)
                     /vntifkey="4"
                     /label=bla\(amp)\sequence
     rep_origin      complement(3242..3242)
                     /vntifkey="33"
                     /label=origin
     CDS             1621..3055
                     /vntifkey="4"
                     /label=1629
     CDS             3..1096
                     /vntifkey="4"
                     /label=lef-2,603
     rep_origin      4992..5447
                     /vntifkey="33"
                     /label=f1\origin
BASE COUNT     1457 a      1226 c      1263 g      1571 t 
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 
     1141 tcaatatata gttgctgatc agatctgtcg actgagcgtc cgtgttcatg atcccgtttt 
     1201 tataacagcc agataaaaat aatcttatca attaagataa aaagataaga ttattaatct 
     1261 aacaacgtgc cttgtgtcac gtaggccaga taacggtcgg gtatataaga cgcctcaacg 
     1321 ctactagtaa atcagtcaca ccaaggcttc aataaggaac acacaagcac catggtccat 
     1381 catcaccacc atcactccgc gggtttggtg cccagaggca gcggcaaaga aacggcggcg 
     1441 gcgaaatttg aacgccaaca catggacagc gctagcggtg gaggtgatga cgacgacaag 
     1501 agcccgggct tctcctcaaa aggcctggat ccgaattcga gctcgaagct tgcggccgca 
     1561 ctcgagcacc accaccacca ccactaacct aggtagctga gcgcatgcaa gctgatccgg 
     1621 gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt 
     1681 gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa 
     1741 tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt 
     1801 aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat 
     1861 ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt 
     1921 tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat 
     1981 gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt 
     2041 taaatagagc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt 
     2101 cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac 
     2161 gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg 
     2221 gaattatttc tgattgcggg cgtttttggg cgggtttcaa tctaactgtg cccgatttta 
     2281 attcagacaa cacgttagaa agcgatggtg caggcggtgg taacatttca gacggcaaat 
     2341 ctactaatgg cggcggtggt ggagctgatg ataaatctac catcggtgga ggcgcaggcg 
     2401 gggctggcgg cggaggcgga ggcggaggtg gtggcggtga tgcagacggc ggtttaggct 
     2461 caaatgtctc tttaggcaac acagtcggca cctcaactat tgtactggtt tcgggcgccg 
     2521 tttttggttt gaccggtctg agacgagtgc gatttttttc gtttctaata gcttccaaca 
     2581 attgttgtct gtcgtctaaa ggtgcagcgg gttgaggttc cgtcggcatt ggtggagcgg 
     2641 gcggcaattc agacatcgat ggtggtggtg gtggtggagg cgctggaatg ttaggcacgg 
     2701 gagaaggtgg tggcggcggt gccgccggta taatttgttc tggtttagtt tgttcgcgca 
     2761 cgattgtggg caccggcgca ggcgccgctg gctgcacaac ggaaggtcgt ctgcttcgag 
     2821 gcagcgcttg gggtggtggc aattcaatat tataattgga atacaaatcg taaaaatctg 
     2881 ctataagcat tgtaatttcg ctatcgttta ccgtgccgat atttaacaac cgctcaatgt 
     2941 aagcaattgt attgtaaaga gattgtctca agctcggatc gatcccgcac gccgataaca 
     3001 agccttttca tttttactac agcattgtag tggcgagaca cttcgctgtc gtcgaggttt 
     3061 aaacgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
     3121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
     3181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     3241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     3301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     3361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     3421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     3481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     3541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     3601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     3661 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     3721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     3781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     3841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     3901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     3961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     4021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     4081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     4141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     4201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     4261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     4321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     4381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     4441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     4501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     4561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 
     4621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     4681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     4741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     4801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     4861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     4921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     4981 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
     5041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
     5101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
     5161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
     5221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
     5281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
     5341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
     5401 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt 
     5461 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgcca 
//